Pacific Blue™ anti-mouse CD90.2 (Thy-1.2) An
- 产品名称：
- Pacific Blue™ anti-mouse CD90.2 (Thy-1.2) An
- 产品类别：
- 抗体
- 产品编号：
- 140305
- 产品应用：
- 140305
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).
- Application References
(PubMed link indicates BioLegend citation) -
- Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
- Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
- Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
- Product Citations
-
- Dalmas E et al. 2017. Immunity. 47(5):928-942 . PubMed
- Khodeer S, et al. 2022. J Cell Sci. 135:. PubMed
- Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
- Mathur S, et al. 2021. JCI Insight. 6:. PubMed
- Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
- Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
- Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
- Deets KA, et al. 2021. Elife. 10:. PubMed
- Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed
- RRID
- AB_10645335 (BioLegend Cat. No. 140305) AB_10641693 (BioLegend Cat. No. 140306)
- Structure
- Ig superfamily, 25-35 kD
- Distribution
-
Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice
- Function
- Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
- Ligand/Receptor
- CD45
- Cell Type
- Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
-
1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.
- Gene ID
- 21838 View all products for this Gene ID
- UniProt
- View information about CD90.2 on UniProt.org
