Brilliant Violet 605™ anti-mouse CD90.2 (Thy
- 产品名称：
- Brilliant Violet 605™ anti-mouse CD90.2 (Thy
- 产品类别：
- 抗体
- 产品编号：
- 140317
- 产品应用：
- 140317
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- ?l size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
- Concentration
- ?g size: 0.2 mg/mL?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).
- Application References
(PubMed link indicates BioLegend citation) -
- Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
- Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
- Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
- Product Citations
-
- Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
- Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
- Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
- Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed
- Cunningham C, et al. 2016. PLoS Pathog. 12: 1005356. PubMed
- Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
- K?chl R, et al. 2020. Elife. 9:00. PubMed
- Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
- Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
- Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
- de Lucía Finkel P, et al. 2021. Sci Rep. 11:12233. PubMed
- McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
- Lima-Junior DS, et al. 2021. Cell. . PubMed
- Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
- Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
- Zeis P, et al. 2020. Immunity. 53:775. PubMed
- Wilmore JR, et al. 2022. Front Immunol. 12:791095. PubMed
- Aggio-Bruce R, et al. 2020. Mol Neurobiol. . PubMed
- Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
- Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
- Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
- Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
- RRID
- AB_11203724 (BioLegend Cat. No. 140317) AB_2650924 (BioLegend Cat. No. 140318)
- Structure
- Ig superfamily, 25-35 kD
- Distribution
-
Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice
- Function
- Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
- Ligand/Receptor
- CD45
- Cell Type
- Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
-
1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.
- Gene ID
- 21838 View all products for this Gene ID
- UniProt
- View information about CD90.2 on UniProt.org
