Brilliant Violet 510™ anti-mouse CD90.2 (Thy
- 产品名称：
- Brilliant Violet 510™ anti-mouse CD90.2 (Thy
- 产品类别：
- 抗体
- 产品编号：
- 140319
- 产品应用：
- 140319
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).
- Application References
(PubMed link indicates BioLegend citation) -
- Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
- Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
- Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
- Product Citations
-
- Georges RO, et al. 2022. Nat Commun. 13:6230. PubMed
- Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
- Vicetti Miguel R, et al. 2016. PLoS One. 11: 0162445. PubMed
- Wang H, et al. 2022. Curr Protoc. 2:e446. PubMed
- Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
- Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
- Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
- Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
- Baerenwaldt A, et al. 2016. J Immunol. 196: 2561 - 2571. PubMed
- Krisna SS, et al. 2021. STAR Protoc. 2:100627. PubMed
- Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
- RRID
- AB_2561395 (BioLegend Cat. No. 140319)
- Structure
- Ig superfamily, 25-35 kD
- Distribution
-
Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice
- Function
- Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
- Ligand/Receptor
- CD45
- Cell Type
- Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
-
1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.
- Gene ID
- 21838 View all products for this Gene ID
- UniProt
- View information about CD90.2 on UniProt.org
