Ultra-LEAF™ Purified anti-human CD1d Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2844	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human CD1d-Fc fusion

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC, IP, Stim, WB, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections¹, Western blotting^1,2, and induction of IL-12 production by crosslinking of CD1d³.

Application References (PubMed link indicates BioLegend citation)

Exley M, et al. 2000. Immunology 100:37. (IHC, WB)
Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB)
Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)

RRID

AB_2814280 (BioLegend Cat. No. 350321) AB_2814281 (BioLegend Cat. No. 350322)

Antigen Details

Structure: MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution: Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells
Function: Present lipid antigens to iNKT cells
Ligand/Receptor: When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens, TCRs
Antigen References: 1. Koch M, et al. 2005. Nat. Immunol. 6:819.
2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010.
3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889.
4. Teige A, et al. 2010. J. Immunol. 185:345.
Gene ID: 912 View all products for this Gene ID
UniProt: View information about CD1d on UniProt.org

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