Alexa Fluor® 700 anti-mouse CD3ε Antib
- 产品名称：
- Alexa Fluor® 700 anti-mouse CD3ε Antib
- 产品类别：
- 抗体
- 产品编号：
- 152315
- 产品应用：
- 152315
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- Mouse T cell receptor.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.
- Application References
(PubMed link indicates BioLegend citation) -
- Miescher GC, et al. 1989. Immunol. Lett. 23:113.
- Product Citations
-
- Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
- Roy R, et al. 2022. Elife. 11:. PubMed
- Poillet-Perez L, et al. 2020. Nat Cancer. 1:923. PubMed
- Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
- Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
- Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
- RRID
- AB_2632712 (BioLegend Cat. No. 152315) AB_2632713 (BioLegend Cat. No. 152316)
- Structure
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
- Distribution
-
Thymocytes (differentiation dependent), mature T cells and NK-T cells.
- Function
- TCR signal transduction, T cell activation and antigen recognition.
- Interaction
- T-cell receptor (TCR).
- Ligand/Receptor
- Peptide antigen/MHC-complex.
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263.
- Gene ID
- 12501 View all products for this Gene ID
- UniProt
- View information about CD3epsilon on UniProt.org
