Alexa Fluor® 594 anti-mouse CD3ε Antib
- 产品名称：
- Alexa Fluor® 594 anti-mouse CD3ε Antib
- 产品类别：
- 抗体
- 产品编号：
- 152317
- 产品应用：
- 152317
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- Mouse T cell receptor.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-F -?Quality tested
3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.
- Application References
(PubMed link indicates BioLegend citation) -
- Miescher GC, et al. 1989. Immunol. Lett. 23:113.
- Product Citations
-
- Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
- Mayes-Hopfinger L, et al. 2021. Nat Commun. 12:4546. PubMed
- RRID
- AB_2715809 (BioLegend Cat. No. 152317) AB_2715810 (BioLegend Cat. No. 152318)
- Structure
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
- Distribution
-
Thymocytes (differentiation dependent), mature T cells and NK-T cells.
- Function
- TCR signal transduction, T cell activation and antigen recognition.
- Interaction
- T-cell receptor (TCR).
- Ligand/Receptor
- Peptide antigen/MHC-complex.
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263.
- Gene ID
- 12501 View all products for this Gene ID
- UniProt
- View information about CD3epsilon on UniProt.org
