Alexa Fluor® 488 anti-mouse CD3ε Antib-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1488	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Syrian Hamster

Immunogen

Mouse T cell receptor.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 ?g/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Application References (PubMed link indicates BioLegend citation)

Miescher GC, et al. 1989. Immunol. Lett. 23:113.

RRID

AB_2783123 (BioLegend Cat. No. 152321) AB_2783124 (BioLegend Cat. No. 152322)

Antigen Details

Structure: Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution: Thymocytes (differentiation dependent), mature T cells and NK-T cells.
Function: TCR signal transduction, T cell activation and antigen recognition.
Interaction: T-cell receptor (TCR).
Ligand/Receptor: Peptide antigen/MHC-complex.
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263.
Gene ID: 12501 View all products for this Gene ID
UniProt: View information about CD3epsilon on UniProt.org

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