Anti-Ubiquitin, 64-76 (free/bound) Antibody (Previ
- 产品名称：
- Anti-Ubiquitin, 64-76 (free/bound) Antibody (Previ
- 产品类别：
- 抗体
- 产品编号：
- 834901
- 产品应用：
- 834901
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
- Application
-
WB - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 - 1:5000 in human, mouse and rat and 1:500-1:2000 in Drosophila.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution range of 1:1000 - 1:5000 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody detects ubiquitin and ubiquitinated paired helical filaments (PHF). It is specific for residues 64-76 of ubiquitin and recognizes free and bound ubiquitin, and native or detergent extracted PHF.
Reactivity to Drosophila was only verified with the purified format.
- Application References
(PubMed link indicates BioLegend citation) -
- Grundke-Iqbal I, et al. 1986. J Biol Chem. 261:6084-6089. (WB)
- Kudo T, et al. 1994. Brain Res. 1:1-7. (ELISA)
- Xu G, et al. 2012. PLoS One 11:e49021. (WB) PubMed
- Wang J, et al. 2003. Hum. Mol. Genet. 21:2753. (WB)
- Bazzaro M, et al. 2006. Cancer Res. 7:3754. (WB) PubMed
- Tabaton M, et al. 1991. Proc. Natl. Acad. Sci. USA 6:2098. (EM, IHC)
- Merz PA, et al. 1992. Am. J. Pathol. 2:343. (EM)
- Product Citations
-
- Bazzaro M, et al. 2006. Cancer Res. 66:3754-3763. PubMed
- Xu G, et al. 2012. PLoS One. 7:e49021. PubMed
- RRID
- AB_2565315 (BioLegend Cat. No. 834901)
- Structure
- Ubiquitin is a 76 amino acid protein with a molecular mass of 8.5 kD.
- Distribution
-
Found in almost all tissues of eukaryotic organisms.
- Function
- Regulatory protein.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
- Molecular Family
- Autophagosome Markers, Protein Modifiers
- Gene ID
- 7316 View all products for this Gene ID
- UniProt
- View information about Ubiquitin, 64-76 on UniProt.org
