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首页 > 抗体蛋白 > 抗体
PE/Cyanine7 anti-human IFN-γ Antibody
产品名称：
PE/Cyanine7 anti-human IFN-γ Antibody
产品类别：
抗体
产品编号：
502528
产品应用：
502528
[价格]
规格 价格 库存
100tests ￥ 3876 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified, native human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ?-producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ?in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
  2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
  3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
  4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
  5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
  6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
  8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
Product Citations
  1. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  2. Luo X, et al. 2020. Front Immunol. 11:623. PubMed
  3. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  4. Witkowski M, et al. 2021. Nature. 600:295. PubMed
  5. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  6. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  7. Kagoya Y, et al. 2018. Nat Med. 24:352. PubMed
  8. Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed
  9. Brembilla N, et al. 2011. PLoS One. 15:18741. PubMed
  10. Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
  11. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  12. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  13. Bastiaens G, et al. 2016. Am J Trop Med Hyg. 94: 663 - 673. PubMed
  14. Jiang J, et al. 2017. Arthritis Res Ther. 10.1186/s13075-017-1261-9. PubMed
  15. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  16. Lim D, et al. 2014. J Immunol. 193:5065. PubMed
  17. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  18. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  19. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  20. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  21. Jiang B, et al. 2014. Cell Immunol. 290:10. PubMed
  22. Leng T, et al. 2019. Cell Rep. 28:3077. PubMed
  23. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  24. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-11056-9. PubMed
  25. Ferreira-Gomes M, et al. 2021. Nat Commun. 12:1961. PubMed
  26. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  27. Trivedi S, et al. 2020. Elife. 9:00. PubMed
RRID
AB_1626154 (BioLegend Cat. No. 502527) AB_2123323 (BioLegend Cat. No. 502528)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

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