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FITC anti-human IFN-γ Antibody
产品名称：
FITC anti-human IFN-γ Antibody
产品类别：
抗体
产品编号：
502505
产品应用：
502505
[价格]
规格 价格 库存
25tests ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified, native human IFN-γ
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
?g sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ?-producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ?in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
  2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
  3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
  4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
  5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
  6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
  8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
Product Citations
  1. Carre C, et al. 2021. iScience. 24:102970. PubMed
  2. Pan YG, et al. 2022. Methods Mol Biol. 2574:31. PubMed
  3. Zhou Y, et al. 2020. Cell Rep. 31:107574. PubMed
  4. Lu T, et al. 2016. PLoS One. 11: 0148044. PubMed
  5. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  6. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  7. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  8. Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed
  9. Shokri MR, et al. 2019. Sci Rep. 9:10007. PubMed
  10. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  11. Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed
  12. Priyanto H, et al. 2021. J Clin Tuberc Other Mycobact Dis. 22:100214. PubMed
  13. Zou F, et al. 2021. Mol Ther. 29:1794. PubMed
  14. Spencer A, et al. 2014. PLoS One. 9:100538. PubMed
  15. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  16. Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed
  17. Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed
  18. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  19. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  20. Yu J, et al. 2019. Oncol Lett. 17:1461. PubMed
  21. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  22. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  23. Hamilton JR, et al. 2021. Cell Reports. 35(9):109207. PubMed
  24. Lu H, et al. 2020. Am J Reprod Immunol. 83:. PubMed
  25. Kessler B, et al. 2017. Sci Rep. 7:42791. PubMed
  26. Pierce CA, et al. 2020. Sci Transl Med. 12:00. PubMed
  27. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  28. Stein RG, et al. 2019. Cancer Res. 79:1507. PubMed
  29. Lo Presti E, et al. 2020. J Leukoc Biol. 108:749. PubMed
  30. Poulsen L 2012. J Immunol. 189:4431. PubMed
  31. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  32. Vacaflores A, et al. 2017. Mol Immunol. 81:1. PubMed
  33. Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
  34. Rochman Y, et al. 2015. PLoS One. 10:122198. PubMed
  35. Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
RRID
AB_315230 (BioLegend Cat. No. 502505) AB_315232 (BioLegend Cat. No. 502507) AB_315231 (BioLegend Cat. No. 502506)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

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