Purified anti-human CD116 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1212	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human GM-CSFRα transfected COS cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IP, WB, IHC-P, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation², Western blotting¹, and immunohistochemical staining of acetone-fixed frozen tissue sections⁴ and paraffin-embedded tissue sections⁵.

Application References (PubMed link indicates BioLegend citation)

Stomski FC, et al. 1998. J. Biol. Chem. 273:1192. (WB)
McClure B, et al. 2001. Blood 98:3165. (IP)
Guthridge MA, et al. 2004. Blood 103:820.
Xiong S, et al. 2013. J. Clin. Invest. 123:4264. (IHC)
Sawada H, et al. 2014. J. Exp. Med. 211:263. (IHC).

Product Citations

Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed

RRID

AB_314568 (BioLegend Cat. No. 305902)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70-85 kD
Distribution: Monocytes, granulocytes, dendritic cells, endothelial precursors
Function: Myeloid hematopoietic cell proliferation, differentiation
Ligand/Receptor: GM-CSF
Cell Type: Dendritic cells, Endothelial cells, Granulocytes, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 1438 View all products for this Gene ID
UniProt: View information about CD116 on UniProt.org

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