HRP anti-β-Amyloid, 17-24 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1416	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residues 17-24 of ? amyloid. The epitope lies within amino acids 18-22 of ? amyloid (VFFAE). 4G8 ?-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms.

This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4).

Additional reported applications (for the relevant formats) include:?immunohistochemical staining on frozen tissue sections (IHC-F) and?immunocytochemistry (ICC)

Application References (PubMed link indicates BioLegend citation)

Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed
Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed
Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875-87. (IHC-P) PubMed
Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed
Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed
Kawarabayashi T, et al. 2001. J Neurosci 21:372 (IP)
Fonte V, et al. 2002. PNAS. 110:4853 (IP)

RRID

AB_2783371 (BioLegend Cat. No. 800719) AB_2783372 (BioLegend Cat. No. 800720)

Antigen Details

Structure

Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.

Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function

The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

APP/β-Amyloid

Antigen References