Biotin anti-β-Amyloid, 17-24 Antibody (Previ-上海雅吉生物科技有限公司

规格	价格	库存
500µL	￥ 15504	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution (no preservatives or carrier proteins).

Preparation

The antibody was purified by affinity chromatography.

Concentration

1 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.

Application

IHC-P - Quality tested
Direct ELISA - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 2.0 ?g/ml is suggested.?For Direct ELISA applications, a concentration range of 0.5 - 2.0 ?g/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residues 17-24 of ? amyloid. The epitope lies within amino acids 18-22 of ? amyloid (VFFAE). 4G8 ?-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms.

This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4).

Additional reported applications (for the relevant formats) include:?immunohistochemical staining on frozen tissue sections (IHC-F) and?immunocytochemistry (ICC)

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed
Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed
Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875-87. (IHC-P) PubMed
Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed
Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed
Kawarabayashi T, et al. 2001. J Neurosci 21:372 (IP)
Fonte V, et al. 2002. PNAS. 110:4853 (IP)

Product Citations

Lam S, et al. 2022. Acta Neuropathol Commun. 10:112. PubMed
Jung ES, et al. 2022. Aging Cell. 21:e13623. PubMed
Townsend M, et al. 2010. J Pharmacol Exp Ther. 333:110:119. PubMed
Chen J, et al. 2019. Int J Mol Med. 44:313. PubMed
Elmer BM, et al. 2019. PLoS One. 14:e0226245. PubMed
Yao Q, et al. 2018. Acta Biochim Biophys Sin (Shanghai). 50(5):447-455. PubMed
Watanabe H, et al. 2012. J Neurosci. 32:5085-5096. PubMed
Hong J, et al. 2016. J Lipid Res. 57: 36 - 45. PubMed
Clayton K, et al. 2021. Mol Neurodegener. 18:16. PubMed
Bales K, et al. 2016. Brain. 139: 563 - 577. PubMed
Luo M, et al. 2022. Genes Dis. 9:1315. PubMed
Forgrave LM, et al. 2022. Clin Chem Lab Med. 60:198. PubMed
Do TM, et al. 2020. Mol Ther Methods Clin Dev. 19:58. PubMed

RRID

AB_2564650 (BioLegend Cat. No. 800704) AB_2564651 (BioLegend Cat. No. 800705) AB_2564649 (BioLegend Cat. No. 800706)

Antigen Details

Structure

Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.

Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function

The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

APP/β-Amyloid

Antigen References