Anti-β-Amyloid, 17-24 Antibody (Previously Co-上海雅吉生物科技有限公司

规格	价格	库存
50µL	￥ 3600	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Preparation

Ascites

Concentration

The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.

Storage & Handling

Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.

Application

IHC-P - Quality tested
Direct ELISA - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested.?For Direct ELISA applications, a dilution range of 1:100 - 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residues 17-24 of ? amyloid. The epitope lies within amino acids 18-22 of ? amyloid (VFFAE). 4G8 ?-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms.

This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4).

Application References (PubMed link indicates BioLegend citation)

Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed
Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed
Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875:87. (IHC-P) PubMed
Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed
Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed

Product Citations

Klakotskaia D, et al. 2018. PLoS One. 8:8755. PubMed
Woelfle S, et al. 2021. Front Cell Neurosci. 15:789903. PubMed
Choi S, et al. 2015. PLoS One. 10: 0143854. PubMed
Taddei RN, et al. 2022. Acta Neuropathol Commun. 10:72. PubMed
Gkanatsiou E, et al. 2021. J Neurochem. 159:258. PubMed
Jiwaji Z, et al. 2022. Nat Commun. 13:135. PubMed
Morales R, et al. 2021. Mol Psychiatry. 26:5690. PubMed
Koronyo-Hamaoui M, et al. 2020. Brain. 143:336. PubMed
Martin L, et al. 2021. Cell Reports. 35(6):109121. PubMed
Chaudry O, et al. 2022. Sci Rep. 12:19129. PubMed
Whitehouse I, et al. 2016. PLoS One. 11: 0159119. PubMed
Mu L, et al. 2022. Int J Mol Sci. 23:. PubMed
Robertson AS, et al. 2017. Neuromuscul Disord. 27:635. PubMed
Gamez N, et al. 2022. Cells. 11:. PubMed
Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed
Schaefer P, et al. 2016. PLoS One. 11:e0168157. PubMed
Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed
Zhang J, et al. 2021. iScience. 24:103327. PubMed
Lessard C, et al. 2015. PLoS One. 10: 0144758. PubMed
Boeddrich A et al. 2018. Cell chemical biology. 26(1):109-120 . PubMed
Tang Z, et al. 2019. Nat Commun. 10:2173. PubMed
Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed
Maxwell AM, et al. 2021. Acta Neuropathol Commun. 9:201. PubMed

RRID

AB_2734547 (BioLegend Cat. No. 800708) AB_2565325 (BioLegend Cat. No. 800709) AB_2565326 (BioLegend Cat. No. 800710) AB_2565324 (BioLegend Cat. No. 800711)

Antigen Details

Structure

Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.

Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function

The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

APP/β-Amyloid

Antigen References