Purified anti-mouse Ly49D Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3516	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation¹ and blocking^{2, 3}of CHO cell or Con A-activated lymphoblast killing by NK cells.

Application References (PubMed link indicates BioLegend citation)

Mason LH, et al. 1998. J. Immunol. 160:4148. (IP)
Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block)
George TC, et al. 1999. J. Immunol. 162:2035. (FC Block)
Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC)
Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)

Product Citations

Goh W, et al. 2020. Cell Rep. 33:108285. PubMed

RRID

AB_10574460 (BioLegend Cat. No. 138302)

Antigen Details

Structure: A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
Distribution: Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.
Function: Plays an important role in activating natural killer (NK) cell cytolytic activity.
Ligand/Receptor: MHC class I alloantigens
Cell Type: NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.
Gene ID: 16635 View all products for this Gene ID
UniProt: View information about Ly49D on UniProt.org

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