Alexa Fluor® 647 anti-mouse Ly49D Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse Ly49D Antibody
- 产品类别：
- 抗体
- 产品编号：
- 138306
- 产品应用：
- 138306
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.
- Application References
(PubMed link indicates BioLegend citation) -
- Mason LH, et al. 1998. J. Immunol. 160:4148. (IP)
- Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block)
- George TC, et al. 1999. J. Immunol. 162:2035. (FC Block)
- Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC)
- Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)
- Product Citations
-
- Wagner JA, et al. 2020. Cell Rep. 31:107720. PubMed
- Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
- RRID
- AB_10574955 (BioLegend Cat. No. 138306)
- Structure
- A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
- Distribution
-
Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.
- Function
- Plays an important role in activating natural killer (NK) cell cytolytic activity.
- Ligand/Receptor
- MHC class I alloantigens
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.
- Gene ID
- 16635 View all products for this Gene ID
- UniProt
- View information about Ly49D on UniProt.org
