Purified anti-mouse MCP-1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 2820	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Hamster

Immunogen

CHO-expressed, recombinant mouse MCP-1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture: The purified 4E2/MCP antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detecting antibody for the detection of mouse MCP-1. The LEAF? purified antibody is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: Western blotting¹. The 4E2/MCP is a non-neutralizing antibody¹.

Application References (PubMed link indicates BioLegend citation)

Luo Y, et al. 1994. J. Immunol. 153:3708.
Luo Y, et al. 1999 J. Immunol. 163:3985.

Product Citations

Yu J, et al. 2011. Eur J Cell Biol. 90:1000. PubMed

RRID

AB_2290924 (BioLegend Cat. No. 506004)

Antigen Details

Structure: CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity: Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors: CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271.
3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.
Regulation: Upregulated by IL-1, TNF-a, PDGF, TGF-?
Gene ID: 20296 View all products for this Gene ID
UniProt: View information about MCP-1 on UniProt.org

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