Ultra-LEAF™ Purified anti-mouse TIGIT (Vstm3
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse TIGIT (Vstm3
- 产品类别：
- 抗体
- 产品编号：
- 156107
- 产品应用：
- 156107
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse TIGIT Tetramers
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
It was reported that this clone can block the interaction between mouse TIGIT and CD155.
- Application References
(PubMed link indicates BioLegend citation) -
- Joller N,?et al.?2011.?J. Immunol.?186:1338.
- RRID
- AB_2810422 (BioLegend Cat. No. 156107) AB_2810423 (BioLegend Cat. No. 156108)
- Structure
- Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.
- Distribution
-
Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.
- Function
- Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.
- Ligand/Receptor
- CD155 (PVR) and CD112 (PVRL2).
- Cell Type
- Dendritic cells, NK cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
-
- Levin SD,?et al.?2011.?Eur. J. Immunol.?41:902.
- Yu X,?et al.?2009.?Nat. Immunol.?10:48.
- Stanietsky N,?et al.?2009.?Proc.?Natl. Acad. Sci.?USA 106:17858
- Gene ID
- 100043314 View all products for this Gene ID
- UniProt
- View information about TIGIT on UniProt.org
