Purified anti-NF-κB p50 Antibody
- 产品名称：
- Purified anti-NF-κB p50 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 616701
- 产品应用：
- 616701
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- recombinant full-length human NF-κB p50
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 ?g per ml. For immunocytochemistry, a concentration range of 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence1 on the Amnis ImageStream 100 Imaging Flow Cytometer.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Application References
(PubMed link indicates BioLegend citation) -
- Huang F, et al. 2007. J. Immunol. 179:6504. (IF) PubMed
- Cao YA, et al. 2008. J. Biol. Chem. 283:15309. PubMed
- Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed.
- Product Citations
-
- Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed
- Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed
- Kao C, et al. 2008. J Biol Chem. 283:15309. PubMed
- Boudesco C, et al. 2018. Blood. 132:510. PubMed
- Huang F, et al. 2007. J Immunol. 179:6504. PubMed
- RRID
- AB_315855 (BioLegend Cat. No. 616701) AB_315856 (BioLegend Cat. No. 616702)
- Structure
- Member of the Rel/dorsal family. NF-κB p50 is an active form, derived from NF-κB p105, a precursor form.
- Distribution
-
Ubiquitously expressed
- Function
- A transcription factor, originally identified as an activator of kappa light chain in B cells
- Interaction
- Interacts with NF-κB p65 and IκB
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review).
2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).
- Regulation
- NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation.
- Gene ID
- 4790 View all products for this Gene ID
- UniProt
- View information about NF-kappaB p50 on UniProt.org
