Ultra-LEAF™ Purified anti-mouse DR3 (TNFRSF2
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse DR3 (TNFRSF2
- 产品类别：
- 抗体
- 产品编号：
- 144411
- 产品应用：
- 144411
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mtnfr25-Ig fusion protein
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Agonist - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
4C12 is an agonistic antibody. It leads to rapid and selective proliferation of pre-existing Tregs in vivo through TCR engagement with MHC class II (MHC-II) and IL-2 signaling. We recommend our Ultra-LEAF? purified 4C12 antibody (Cat. No. 144411 and 144412) for agonist or in vivo studies.
- Application References
(PubMed link indicates BioLegend citation) -
- Fang L, et al. 2008. J. Exp. Med. 205:1037.
- Wolf D, et al. 2012. Transplantation. 94:569. PubMed
- Schreiber TH, et al. 2012. J. Immunol.?189:3311.
- Longman RS, et al. 2014. J Exp Med. 211:1571. PubMed
- Product Citations
-
- Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
- RRID
- AB_2800671 (BioLegend Cat. No. 144411) AB_2800672 (BioLegend Cat. No. 144412)
- Structure
- 55 kD glycoprotein, contains one death domain and four TNFR-Cys repeats
- Distribution
-
CD4, CD8, NKT and Tregs at low level
- Function
- Plays a role in lymphocyte homeostasis, induces NFKB activation and apoptosis
- Ligand/Receptor
- TL1-A
- Cell Type
- NKT cells, T cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
- Taraban VY, et al. 2011. J. Clin. Invest. 121:463.
- Slebioda TJ, et al. 2011. Eur. J. Immunol. 41:2606.
- Schreiber TH, et al. 2010. J. Clin. Invest. 120:3629.
- Reddy PB, et al. 2012. J. Virol. 2012. [Epub].
- Gene ID
- 85030 View all products for this Gene ID
- UniProt
- View information about DR3 on UniProt.org
