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Purified anti-human CD137 (4-1BB) Antibody
产品名称:
Purified anti-human CD137 (4-1BB) Antibody
产品类别:
抗体
产品编号:
309802
产品应用:
309802
[价格]
规格 价格 库存
100µg ¥ 1572 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
  2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
  3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
  4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations
  1. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  3. Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed
  4. Sun R, et al. 2022. Cells. 11:. PubMed
  5. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  6. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  7. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  8. Tang JS, et al. 2020. Food Funct. 11:5782. PubMed
  9. Ichikawa J, et al. 2020. Clin Cancer Res. 26:3384. PubMed
  10. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
  11. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  12. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314781 (BioLegend Cat. No. 309802)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand
Cell Type
T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org

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