PE/Cyanine7 anti-human CD137 (4-1BB) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1836	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Ectodomain of recombinant human 4-1BB fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,4, inhibition of cytokine production^2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)

Product Citations

Chacon J, et al. 2015. Clin Cancer Res. 21:611. PubMed
Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
Rodriguez-García A, et al. 2020. Mol Ther. 28:548. PubMed
Tischer S, et al. 2012. Int Immunol. 24:561. PubMed
Afanasiev O, et al. 2013. Clin Cancer Res. 19:5351. PubMed
Sandt C, et al. 2016. J Virol. 90: 1009 - 1022. PubMed
Hodge G, et al. 2013. J Heart Lung Transplant. 1053:1357. PubMed
Kilpelainen A, et al. 2022. Front Immunol. 13:815041. PubMed
Gao Y, et al. 2022. Immunity. 55:1732. PubMed
Thieme CJ, et al. 2020. Cell Rep Med. 1:100092. PubMed
Menges D, et al. 2022. Nat Commun. 13:4855. PubMed
Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
Lecciso M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01918. PubMed

RRID

AB_2287731 (BioLegend Cat. No. 309817) AB_2207741 (BioLegend Cat. No. 309818)

Antigen Details

Structure: TNFR superfamily, type I transmembrane protein, 30 kD
Distribution: Activated T cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB ligand
Cell Type: T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 3604 View all products for this Gene ID
UniProt: View information about CD137 on UniProt.org

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