Alexa Fluor® 647 anti-human CD137 (4-1BB) Anti-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1764	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Ectodomain of recombinant human 4-1BB fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,4, inhibition of cytokine production^2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References (PubMed link indicates BioLegend citation)

Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)

Product Citations

Geuijen C, et al. 2021. Nat Commun. 12:4445. PubMed
Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed
Charmetant X, et al. 2022. Sci Transl Med. :eabl6141. PubMed
Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed
Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed

RRID

AB_2566257 (BioLegend Cat. No. 309823) AB_2566258 (BioLegend Cat. No. 309824)

Antigen Details

Structure: TNFR superfamily, type I transmembrane protein, 30 kD
Distribution: Activated T cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB ligand
Cell Type: T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 3604 View all products for this Gene ID
UniProt: View information about CD137 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们