Purified anti-eEF2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µL	￥ 1416	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Partial recombinant human eEF2 protein

Formulation

Phosphate-buffered solution, 0.5% BSA, 0.09% NaN₃, bottle antibody at 0.1 mg/mL.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.1 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested
IHC-P, ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:8000 dilution. For immunocytochemistry, a dilution range of 1:100 is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution range of 1:200 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When used for western blotting, 4B3-G7-H5 dilutions exceeding 1:8000 may produce non-specific binding.

Clone 4B3-G7-H5 was tested for ICC using the following fixation-permeabilization methods: 4% PFA + Triton X-100, 4% PFA + methanol, and methanol-only. Of these methods, methanol-only produced the most robust eEF2 staining.

BioLegend offers two clones, W19410A and 4B3-G7-H5, that detect eEF2.

WB: Both W19410A and 4B3-G7-H5?detected eEF2 with a strong signal, but W19410A was more selective for eEF2. Both clones produced a stronger signal than CST’s antibody when the latter was used at the manufacturer’s recommended dilution.

ICC: W19410A and 4B3-G7-H5 performed similarly for the application.

IP: W19410A passed and 4B3-G7-H5 failed application testing.

IHC-P: W19410A and 4B3-G7-H5 both passed for the application, with 4B3-G7-H5 showing better selectivity.

RRID