PE anti-mouse CD197 (CCR7) Antibody
- 产品名称：
- PE anti-mouse CD197 (CCR7) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 120105
- 产品应用：
- 120105
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CCR7 transfected RBL-2H3 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per 106cells in 100 ?l staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.
Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).
- Application References
(PubMed link indicates BioLegend citation) -
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- Product Citations
-
- Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
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- RRID
- AB_389357 (BioLegend Cat. No. 120105) AB_389358 (BioLegend Cat. No. 120106)
- Structure
- Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
- Distribution
-
Hematopoietic stem cells, T subsets, mature dendritic cells
- Function
- Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
- Ligand/Receptor
- MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
- Cell Type
- Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.
- Gene ID
- 12775 View all products for this Gene ID
- UniProt
- View information about CD197 on UniProt.org
