APC/Fire™ 810 anti-mouse CD197 (CCR7) Antibo
- 产品名称：
- APC/Fire™ 810 anti-mouse CD197 (CCR7) Antibo
- 产品类别：
- 抗体
- 产品编号：
- 120135
- 产品应用：
- 120135
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CCR7 transfected RBL-2H3 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire? 810 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire? 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire? 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.
Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).
- Application References
(PubMed link indicates BioLegend citation) -
- Ohl L, et al. 2004. Immunity 21:279.
- Ritter U, et al. 2004. J. Leukocyte Biol. 76:472.
- Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC)
- Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed
- Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed
- Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC)
- Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC)
- Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC)
- Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed
- Kang SG, et al. 2007. J. Immunol. 179:3724.
- Mao A et al. 2005. J. Immunol. 175:5146. PubMed
- Allende ML, et al. 2008. FASEB J. 22:307. PubMed
- Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed
- Chen H, et al. 2005. J. Immunol. 175:591. PubMed
- Florido M, et al. 2009. Immunobiology. 214:643. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- RRID
- AB_2910289 (BioLegend Cat. No. 120135)
- Structure
- Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
- Distribution
-
Hematopoietic stem cells, T subsets, mature dendritic cells
- Function
- Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
- Ligand/Receptor
- MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.
- Gene ID
- 12775 View all products for this Gene ID
- UniProt
- View information about CD197 on UniProt.org
