Purified anti-T-bet Antibody
- 产品名称：
- Purified anti-T-bet Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644802
- 产品应用：
- 644802
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB, ICFC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting and intracelluar immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For Western blotting, the suggested use is 1.0 to 2.0 ?g per ml. For flow cytometric staining, the suggested use of this reagent is 1.0 ?g per million cells in a staining volume of 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
- Stankovic S, et al. 2015. J Immunol. 195: 4892 - 4899. PubMed
- Seifert HA, et al. 2018. Metab Brain Dis. 33:1599. PubMed
- Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
- Wang D, et al. 2018. Immunity. 48:659. PubMed
- Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
- Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
- Chen J, et al. 2020. J Immunol. 204:2076. PubMed
- Wang Y, et al. 2021. Cancer Cell. :. PubMed
- Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
- Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
- Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
- Hsieh C, et al. 2012. J Pharmacol Exp Ther. 343:125. PubMed
- Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
- Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
- O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
- Yang K, et al. 2017. Nature. 548:602. PubMed
- Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
- Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
- Ejima R, et al. 2021. Nutrients. 13:. PubMed
- Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
- Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
- Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
- Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
- Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
- Wang D, et al. 2022. EMBO Rep. 23:e53691. PubMed
- Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
- Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
- Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
- Dey P, et al. 2020. Cancer Discov. 10:608. PubMed
- Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
- Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
- Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
- RRID
- AB_1595608 (BioLegend Cat. No. 644801) AB_1595503 (BioLegend Cat. No. 644802)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
