Purified anti-T-bet (Maxpar® Ready) Antibody
- 产品名称：
- Purified anti-T-bet (Maxpar® Ready) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644825
- 产品应用：
- 644825
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
CyTOF? - Verified
- Recommended Usage
This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Additional Product Notes
Maxpar? is a registered trademark of Standard BioTools Inc.
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
- Wei SC, et al. 2019. Immunity. 50:1084. PubMed
- Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
- Gruber CN, et al. 2020. Cell. 183:982. PubMed
- McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
- Gubin MM, et al. 2018. Cell. 175:1014. PubMed
- Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
- Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
- Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
- RRID
- AB_2563788 (BioLegend Cat. No. 644825)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
