PerCP/Cyanine5.5 anti-T-bet Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-T-bet Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644805
- 产品应用：
- 644805
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 ?g per million cells in a volume of 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Frasca D, et al. 2019. PLoS One. 14:e0219545. PubMed
- Amelio P, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005817. PubMed
- Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
- Huanosta-Murillo E, et al. 2021. Front Immunol. 12:668369. PubMed
- Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
- Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
- Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed
- Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
- Webb ER, et al. 2022. iScience. 25:104995. PubMed
- Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
- Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
- Nabatanzi R, et al. 2021. BMC Immunol. 22:59. PubMed
- Hurton L, et al. 2016. Proc Natl Acad Sci U S A. 113: E7788 - E7797. PubMed
- Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
- Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
- Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
- Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
- Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
- RRID
- AB_1595593 (BioLegend Cat. No. 644805) AB_1595488 (BioLegend Cat. No. 644806)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
