PE/Dazzle™ 594 anti-T-bet Antibody
- 产品名称：
- PE/Dazzle™ 594 anti-T-bet Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644827
- 产品应用：
- 644827
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 ?g per million cells in a staining volume of 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Fitzgerald AA, et al. 2021. J Immunother Cancer. 9:. PubMed
- Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
- Stokes J, et al. 2020. Oncoimmunology. 9:1758011. PubMed
- Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
- Harper JL, et al. 2020. Nat Med. 519:26. PubMed
- Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
- Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed
- Baja?a S, et al. 2022. iScience. 25:103732. PubMed
- RRID
- AB_2565676 (BioLegend Cat. No. 644827) AB_2565677 (BioLegend Cat. No. 644828)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
