PE/Cyanine7 anti-T-bet Antibody
- 产品名称：
- PE/Cyanine7 anti-T-bet Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644823
- 产品应用：
- 644823
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
- Wilfahrt D, et al. 2021. Elife. 10:. PubMed
- Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
- Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
- Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
- Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
- Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
- Andrews SF et al. 2019. Immunity. 51(2):398-410 . PubMed
- Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
- Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
- Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
- Hong MJ, et al. 2018. Mucosal Immunol. 11:894. PubMed
- Ly A, et al. 2020. Cell Reports. 29(8):2257-2269.e6.. PubMed
- Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
- Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
- Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
- Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
- Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed
- Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
- Chen RJ, et al. 2022. iScience. 25:105595. PubMed
- Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
- Sokal A, et al. 2021. Cell. 184(5):1201-1213.e14. PubMed
- Sjaastad FV, et al. 2020. Front Immunol. 11:1786. PubMed
- Bhattacharya P, et al. 2020. Elife. 9:00. PubMed
- Joubert IA, et al. 2020. Vaccine. 38:1015. PubMed
- Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
- Hernndez DC, et al. 2021. Immunity. :. PubMed
- Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
- Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
- Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed
- Sharma M, et al. 2019. Immunometabolism. 1. PubMed
- Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
- Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
- Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
- RRID
- AB_2561760 (BioLegend Cat. No. 644823) AB_2561761 (BioLegend Cat. No. 644824)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
