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PE anti-T-bet Antibody
产品名称：
PE anti-T-bet Antibody
产品类别：
抗体
产品编号：
644809
产品应用：
644809
[价格]
规格 价格 库存
25µg ￥ 2568 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 ?g per million cells in a staining volume of 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  2. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  3. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  4. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  5. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  6. Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed
  7. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  8. Tauriainen J, et al. 2017. Sci Rep. 7:40354. PubMed
  9. Chen D, et al. 2018. Nat Commun. 9:873. PubMed
  10. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  11. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  12. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  13. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  14. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  15. Werbner M, et al. 2019. mSystems. 4:e00292-18. PubMed
  16. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  17. Mao A,et al. 2017. Nat Commun. . 10.1038/s41467-017-00882-0. PubMed
  18. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  19. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  20. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  21. Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed
  22. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  23. Śledzińska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed
  24. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  25. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  26. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  27. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  28. Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed
  29. Pieper R, et al. 2020. Gut Microbes. 12:1782163. PubMed
RRID
AB_2028583 (BioLegend Cat. No. 644809) AB_2200542 (BioLegend Cat. No. 644810)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

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