Pacific Blue™ anti-T-bet Antibody
- 产品名称：
- Pacific Blue™ anti-T-bet Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644807
- 产品应用：
- 644807
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 ?g per million cells in a volume of 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Liu F, et al. 2016. PLoS Pathog. 12: 1005663. PubMed
- Kalekar L, et al. 2016. Nat Immunol. 17:304-314. PubMed
- Dimova T, et al. 2015. Proc Natl Acad Sci U S A. 112:556. PubMed
- Bellavance E, et al. 2011. J Immunol. 186:3309. PubMed
- Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
- Ma C, et al. 2018. Science. 360:eaan5931. PubMed
- Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
- Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
- Domingo–Gonzalez R, et al. 2020. eLife. 9:e56890.. PubMed
- Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
- Rosário A, et al. 2012. J Immunol. 188:1178. PubMed
- Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed
- Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
- RRID
- AB_1595586 (BioLegend Cat. No. 644807) AB_1595479 (BioLegend Cat. No. 644808)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
