FITC anti-T-bet Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1980	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation² and immunofluorescence microscopy³.

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References (PubMed link indicates BioLegend citation)

Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.

Product Citations

Smith C, et al. 2012. Blood. 120:3237. PubMed
Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed
Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
Dayoub O, et al. 2017. Sci Rep. 10.1038/s41598-017-09933-4. PubMed
Islas-Vazquez L, et al. 2020. Biology (Basel). 9:00. PubMed
Blom L, et al. 2011. PLoS One. 6:e21695. PubMed
Bhattacharya P, et al. 2020. Elife. 9:00. PubMed
Okamoto Y, et al. 2013. Int Immunopharmacol. 17:140. PubMed
Hoffmann M, et al. 2016. PLoS Pathog. 12: 1005661. PubMed
Ostadkarampour M, et al. 2016. PLoS One. 11:e0164751. PubMed
Naluyima P, et al. 2019. J Immunol. 203:2210. PubMed
Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed

RRID

AB_2287097 (BioLegend Cat. No. 644811) AB_2200540 (BioLegend Cat. No. 644812)

Antigen Details

Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009 View all products for this Gene ID
UniProt: View information about T-bet on UniProt.org

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