Direct-Blot™ HRP anti-T-bet Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µL	￥ 4080	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation² and immunofluorescence microscopy³.

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References (PubMed link indicates BioLegend citation)

Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.

RRID

AB_2572010 (BioLegend Cat. No. 644831)

Antigen Details

Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009 View all products for this Gene ID
UniProt: View information about T-bet on UniProt.org

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