Brilliant Violet 421™ anti-T-bet Antibody
- 产品名称：
- Brilliant Violet 421™ anti-T-bet Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644815
- 产品应用：
- 644815
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- ?g size: 0.2 mg/mL?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤ 0.4 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
- Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
- Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
- Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed
- Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed
- Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
- McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
- Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
- Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
- Aalderen M, et al. 2015. J Virol. 89:5110. PubMed
- Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
- Wang HQ, et al. 2021. Cancer Research. 81(11):3079-3091. PubMed
- Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
- Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed
- Singh PP, et al. 2021. Ocul Surf. 21:271. PubMed
- Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
- Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed
- Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
- Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
- Lorentsen KJ, et al. 2018. Nat Commun. 9:1679. PubMed
- Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed
- Schaik B, et al. 2015. J Virol. 89:568. PubMed
- Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
- Hombrink P, et al. 2016. Nat Immunol. 17:1467-1478. PubMed
- Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
- Phalke SP, et al. 2019. PLoS One. 14:e0218827. PubMed
- Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
- Cheng J, et al. 2022. iScience. 25:103588. PubMed
- Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
- Collins N, et al. 2020. Cell. 178(5):1088-1101.e15.. PubMed
- Berrien–Elliott MM, et al. 2020. Immunity. 51(3):479-490. PubMed
- Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
- Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
- Aalderen M, et al. 2013. J Virol. 87:10263. PubMed
- Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
- Goc J, et al. 2021. Cell. :. PubMed
- Song W, et al. 2022. Immunity. 55:290. PubMed
- Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
- Shao TY et al. 2019. Cell host & microbe. 25(3):404-417 . PubMed
- RRID
- AB_10896427 (BioLegend Cat. No. 644815) AB_2686976 (BioLegend Cat. No. 644832) AB_10959653 (BioLegend Cat. No. 644816)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
