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APC anti-T-bet Antibody
产品名称：
APC anti-T-bet Antibody
产品类别：
抗体
产品编号：
644814
产品应用：
644814
[价格]
规格 价格 库存
100µg ￥ 4968 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  2. Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed
  3. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  4. Kaur K, et al. 2021. Cell Rep. 37:110178. PubMed
  5. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  6. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  7. Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  8. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  9. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  10. Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed
  11. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  12. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  13. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  14. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  15. Woodruff M, et al. 2020. medRxiv. . PubMed
  16. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  17. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  18. Wagner A, et al. 2021. Cell. . PubMed
  19. LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed
  20. Lu Y, et al. 2018. Br J Pharmacol. 175:1085. PubMed
  21. Giampazolias E, et al. 2021. Cell. . PubMed
  22. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  23. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  24. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  25. Cao Y, et al. 2019. Nat Commun. 10:1280. PubMed
  26. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  27. Kumar R, et al. 2020. Cell Reports. 30(8):2512-2525. PubMed
RRID
AB_10896913 (BioLegend Cat. No. 644813) AB_10901173 (BioLegend Cat. No. 644814)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

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