Alexa Fluor® 647 anti-T-bet Antibody
- 产品名称：
- Alexa Fluor® 647 anti-T-bet Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644803
- 产品应用：
- 644803
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 ?g per million cells in a volume of 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
- Wang D, et al. 2018. Immunity. 48:659. PubMed
- Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
- Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
- Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
- Soderquest K, et al. 2017. PLoS Genet. 13(2):e1006587. PubMed
- Vogel AB, et al. 2021. Nature. 592:283. PubMed
- Camell CD, et al. 2020. Cell Metabolism. 30(6):1024-1039.e6.. PubMed
- Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
- Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
- Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
- Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
- Doss PMIA, et al. 2021. Cell Reports. 34(10):108833. PubMed
- Wieten R, et al. 2016. PLoS One. 11: 0149871. PubMed
- Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
- Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
- Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
- Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
- RRID
- AB_1595573 (BioLegend Cat. No. 644803) AB_1595466 (BioLegend Cat. No. 644804)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
