Alexa Fluor® 594 anti-T-bet Antibody
- 产品名称：
- Alexa Fluor® 594 anti-T-bet Antibody
- 产品类别：
- 抗体
- 产品编号：
- 644833
- 产品应用：
- 644833
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml (1:100-1:250 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
- Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
- RRID
- AB_2728473 (BioLegend Cat. No. 644833) AB_2728474 (BioLegend Cat. No. 644834)
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
-
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.
- Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
