Ultra-LEAF™ Purified anti-human CD213a2 (IL1
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD213a2 (IL1
- 产品类别：
- 抗体
- 产品编号：
- 360303
- 产品应用：
- 360303
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human IL-13R alpha 2 Fc chimera
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
ELISA, IHC-F, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for relevant formats) include: ELISA1, immunohistochemistry staining of frozen tissue sections1, and blocking (competes with IL-13 for binding).
- Application References
(PubMed link indicates BioLegend citation) -
- Balyasnikova IV, et al. 2012. J. Biol. Chem. 287:30215. (ELISA, IHC, Block)
- RRID
- AB_2562511 (BioLegend Cat. No. 360303)
- Structure
- Member of the hematopoietin family, type I transmembrane protein, contains 1 fibronectin type III domain
- Distribution
-
Activated B cells, fibroblasts, smooth muscle cells, keratinocytes
- Function
- Possible decoy receptor, antagonist of CD213a1, internalization of IL-13
- Ligand/Receptor
- IL-13
- Cell Type
- B cells, Fibroblasts
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Mitchell J, et al. 2010. Curr. Opin. Investig Drugs. 11:527.
2. Strober W, et al. 2009. Curr. Mol. Med. 9:740.
3. Tabata Y and Khurana Hershey GK. et al. 2007. Curr. Allergy Asthma Rep. 7:338.
- Gene ID
- 3598 View all products for this Gene ID
- UniProt
- View information about CD213a2 on UniProt.org
