Alexa Fluor® 488 anti-Tau, 1-100 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-Tau, 1-100 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 816607
- 产品应用：
- 816607
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against full-length human tau protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
IHC, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 – 5 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
?
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
This antibody is reactive to amino acid residues 1-100 of human Tau. The epitope lies within amino acids 6-18. This clone is specific for all six isoforms of human tau protein.
This antibody clone has been reported for use on IHC of 4% PFA-fixed free floating sections5.
This antibody is exclusively provided by BioLegend.
- RRID
- AB_2721371 (BioLegend Cat. No. 816607) AB_2721372 (BioLegend Cat. No. 816608)
- Structure
- Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
- Distribution
-
Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane and cytosol.
- Function
- Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Interaction
- Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Tau
- Antigen References
-
- Augustinack JC,?et al.?2002.?Acta Neuropath. 103(1):26-35.
- Seubert P,?et al.?1995.?J Biol Chem.?270(32):18917-22.
- Dong Y,?et al. 2012.?PLoS One. 7(6):e39386.
- Simic G, et al. 2016. Biomolecules. 6(1):6.
- Dai CL, et al. 2017. Alzheimers Res Ther. 9(1):1. (IHC, WB) [PubMed]
- Baazaoui N, et al. 2017. Alzheimers Res Ther. 9:45. ( WB) [PubMed]
- Dai CL, et al. 2015. J neural Transm (Vienna). 122:607.
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau, 1-100 on UniProt.org
