Ultra-LEAF™ Purified anti-mouse CD106 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
1mg	￥ 8124	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse preadipose cell line PA6

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining^2,3,5-7 of acetone-fixed frozen sections, blocking^4,5,8 of ligand binding in vitro and in vivo,?immunoprecipitation¹?, and spacial biology (IBEX)^11,12. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References (PubMed link indicates BioLegend citation)

Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2819809 (BioLegend Cat. No. 105727) AB_2819810 (BioLegend Cat. No. 105728)

Antigen Details

Structure: Ig superfamily, 47 kD
Distribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
Function: Adhesion
Ligand/Receptor: VLA-4 (α₄/β₁ integrin) and LPAM-1 (α₄/β₇ integrin)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.
Gene ID: 22329 View all products for this Gene ID
UniProt: View information about CD106 on UniProt.org

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