PE/Cyanine7 anti-mouse CD106 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1692	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse preadipose cell line PA6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining^2,3,5-7 of acetone-fixed frozen sections, blocking^4,5,8 of ligand binding in vitro and in vivo,?immunoprecipitation¹?, and spacial biology (IBEX)^11,12. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Wosczyna MN, et al. 2021. Cell Stem Cell. 28(7):1323-1334.e8. PubMed
Wosczyna MN et al. 2019. Cell Rep. 27(7):2029-2035 . PubMed
Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
Baker N, et al. 2022. Cell Stem Cell. 29:1315. PubMed
Limbad C, et al. 2022. iScience. 25:103848. PubMed
Liu L et al. 2018. Cell stem cell. 23(4):544-556 . PubMed
Li Y, et al. 2021. STAR Protocols. 2(3):100751. PubMed
Prados A, Kollias G, Koliaraki V 2016. Sci Rep. 6: 33027. PubMed

RRID

AB_2214047 (BioLegend Cat. No. 105719) AB_2214046 (BioLegend Cat. No. 105720)

Antigen Details

Structure: Ig superfamily, 47 kD
Distribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
Function: Adhesion
Ligand/Receptor: VLA-4 (α₄/β₁ integrin) and LPAM-1 (α₄/β₇ integrin)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.
Gene ID: 22329 View all products for this Gene ID
UniProt: View information about CD106 on UniProt.org

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