Pacific Blue™ anti-mouse CD106 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3516	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse preadipose cell line PA6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining^2,3,5-7 of acetone-fixed frozen sections, blocking^4,5,8 of ligand binding in vitro and in vivo,?immunoprecipitation¹?, and spacial biology (IBEX)^11,12. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References (PubMed link indicates BioLegend citation)

Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed

RRID

AB_2304290 (BioLegend Cat. No. 105722)

Antigen Details

Structure: Ig superfamily, 47 kD
Distribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
Function: Adhesion
Ligand/Receptor: VLA-4 (α₄/β₁ integrin) and LPAM-1 (α₄/β₇ integrin)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.
Gene ID: 22329 View all products for this Gene ID
UniProt: View information about CD106 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们