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Alexa Fluor® 647 anti-mouse CD106 Antibody
产品名称：
Alexa Fluor® 647 anti-mouse CD106 Antibody
产品类别：
抗体
产品编号：
105711
产品应用：
105711
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse preadipose cell line PA6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?L?volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 2.5 - 10 ?g/mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo,?immunoprecipitation1?, and spacial biology (IBEX)11,12. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
  2. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  3. Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
  4. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  5. Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
  6. Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
  7. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  8. Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
  9. Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
  10. Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
  11. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  12. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed
  2. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  3. Adamiak M, et al. 2015. Leukemia. . PubMed
  4. Sung SJ, et al. 2020. Bio Protoc. 10:e3663. PubMed
  5. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  6. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  7. Nakagawa R, et al. 2013. J Immunol. 190:3309. PubMed
  8. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  9. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  10. Bolkhovitina EL, et al. 2022. Int J Mol Sci. 23:. PubMed
  11. Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed
  12. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  13. Armaka M, et al. 2018. Nat Commun. 9:618. PubMed
  14. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
RRID
AB_493430 (BioLegend Cat. No. 105711) AB_493429 (BioLegend Cat. No. 105712)

Antigen Details

Structure
Ig superfamily, 47 kD
Distribution

Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Adhesion
Ligand/Receptor
VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Gene ID
22329 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org

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