Alexa Fluor® 594 anti-mouse CD106 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3036	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse preadipose cell line PA6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining^2,3,5-7 of acetone-fixed frozen sections, blocking^4,5,8 of ligand binding in vitro and in vivo,?immunoprecipitation¹?, and spacial biology (IBEX)^11,12. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References (PubMed link indicates BioLegend citation)

Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Asare Y, et al. 2020. Circ Res. 127:811. PubMed

RRID

AB_2563962 (BioLegend Cat. No. 105724)

Antigen Details

Structure: Ig superfamily, 47 kD
Distribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
Function: Adhesion
Ligand/Receptor: VLA-4 (α₄/β₁ integrin) and LPAM-1 (α₄/β₇ integrin)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.
Gene ID: 22329 View all products for this Gene ID
UniProt: View information about CD106 on UniProt.org

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