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Purified anti-rat CD80 Antibody
产品名称:
Purified anti-rat CD80 Antibody
产品类别:
抗体
产品编号:
200202
产品应用:
200202
[价格]
规格 价格 库存
500µg ¥ 3096 1

产品详情

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HTLV-1-transformed Lewis-S1 rat T cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 200208).

Application References

(PubMed link indicates BioLegend citation)
  1. Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block)
  2. Bluestone, J.A., 1995. Immunity 2:555. (IP, Block)
  3. Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
  4. Ho, CH., et al. 2007. J. Virol. 81:4837. PubMed
  5. Ho, C., et al. 2009. Carcinogenesis 30:1443. PubMed
Product Citations
  1. Ho C, et al. 2009. Carcinogenesis. 30:1443. PubMed
  2. Ho C, et al. 2007. J Virol. 81:4837. PubMed
RRID
AB_313843 (BioLegend Cat. No. 200202)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Antigen presenting cells, activated B cells and T cells

Function
T and B cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561.
3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.

Gene ID
25408 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

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