Purified anti-rat CD80 Antibody
- 产品名称:
- Purified anti-rat CD80 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 200202
- 产品应用:
- 200202
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HTLV-1-transformed Lewis-S1 rat T cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 200208).
- Application References
(PubMed link indicates BioLegend citation) -
- Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block)
- Bluestone, J.A., 1995. Immunity 2:555. (IP, Block)
- Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
- Ho, CH., et al. 2007. J. Virol. 81:4837. PubMed
- Ho, C., et al. 2009. Carcinogenesis 30:1443. PubMed
- Product Citations
-
- Ho C, et al. 2009. Carcinogenesis. 30:1443. PubMed
- Ho C, et al. 2007. J Virol. 81:4837. PubMed
- RRID
- AB_313843 (BioLegend Cat. No. 200202)
- Structure
- Ig superfamily, 60 kD
- Distribution
-
Antigen presenting cells, activated B cells and T cells
- Function
- T and B cell costimulation
- Ligand/Receptor
- CD28, CD152 (CTLA-4)
- Cell Type
- Antigen-presenting cells, B cells, T cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561.
3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.
- Gene ID
- 25408 View all products for this Gene ID
- UniProt
- View information about CD80 on UniProt.org