PE anti-rat CD80 Antibody
- 产品名称：
- PE anti-rat CD80 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 200205
- 产品应用：
- 200205
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HTLV-1-transformed Lewis-S1 rat T cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining3 of acetone-fixed frozen sections. The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200208).
- Application References
(PubMed link indicates BioLegend citation) -
- Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block)
- Bluestone, J.A., 1995. Immunity 2:555. (IP, Block)
- Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
- Ho, CH., et al. 2007. J. Virol. 81:4837. PubMed
- Ho, C., et al. 2009. Carcinogenesis 30:1443. PubMed
- Tracey Q, et al. 2012. PLoS One. 7:e42662. PubMed.
- Product Citations
-
- Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
- Wu H, et al. 2016. Sci Rep. 6: 24548. PubMed
- Sawanobori Y, et al. 2021. Histochem Cell Biol. 156:133. PubMed
- Lin ZH, et al. 2018. Front Cell Neurosci. 0.729861111. PubMed
- Li B, et al. 2020. Cell Mol Immunol. 1.386111111. PubMed
- Treacy O, et al. 2012. PLoS One. 7:e42662. PubMed
- RRID
- AB_2260182 (BioLegend Cat. No. 200205)
- Structure
- Ig superfamily, 60 kD
- Distribution
-
Antigen presenting cells, activated B cells and T cells
- Function
- T and B cell costimulation
- Ligand/Receptor
- CD28, CD152 (CTLA-4)
- Cell Type
- Antigen-presenting cells, B cells, T cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561.
3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.
- Gene ID
- 25408 View all products for this Gene ID
- UniProt
- View information about CD80 on UniProt.org
