Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation¹⁸. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶, immunoprecipitation³, stimulation of NK cell proliferation⁴, blocking of phagocytosis⁵, and blocking of immunoglobulin binding to FcγRIII^7,8. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
10. Smed-S?rensen A, et al. 2008. Blood 111:5037. (Block) PubMed
11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

• Batch-to-batch consistency
• Material traceability
• Documented procedures
• Documented employee training
• Equipment maintenance and monitoring records
• Lot-specific certificates of analysis
• Quality audits per ISO 13485:2016
• QA review of released products

NK cells, activated monocytes, macrophages, neutrophils

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.