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APC/Cyanine7 anti-human CD16 Antibody
产品名称：
APC/Cyanine7 anti-human CD16 Antibody
产品类别：
抗体
产品编号：
302017
产品应用：
302017
[价格]
规格 价格 库存
25tests ￥ 1668 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-S?rensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Jundi B, et al. 2021. JCI Insight. 6:e148866. PubMed
  2. Lee Y, et al. 2020. J Innate Immun. 1:. PubMed
  3. Lo MW, et al. 2022. Clin Transl Immunology. 11:e1413. PubMed
  4. Masuta Y, et al. 2022. iScience. 25:105085. PubMed
  5. Bekere I, et al. 2021. PLoS Pathog. 17:e1010074. PubMed
  6. Wiernik A, et al. 2013. Clin Cancer Res. 19:3844. PubMed
  7. Bratcher P, et al. 2016. J Cystic Fibrosis. 15: 67-73. PubMed
  8. Hahn J, et al. 2019. Redox Biol. 26:101279. PubMed
  9. Hydes T, et al. 2017. Immun Inflamm Dis. 10.1002/iid3.190. PubMed
  10. Nie Y, et al. 2022. Virol J. 19:68. PubMed
  11. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  12. Ong SM, et al. 2019. Front Immunol. 10:1761. PubMed
  13. Richert-Spuhler LE, et al. 2021. Cell Reports Medicine. 2(6):100322. PubMed
  14. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  15. McCracken M, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006487. PubMed
  16. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  17. Gleason M, et al. 2014. Blood. 123:3016. PubMed
  18. Hejazi M, et al. 2022. Front Immunol. 12:798087. PubMed
  19. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  20. Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
  21. Ehlers L, et al. 2021. The FASEB Journal. 35(7):e21684. PubMed
  22. B?cker-Koduah P, et al. 2020. Ann Clin Transl Neurol. 1.422222222. PubMed
  23. Dobbs KR, et al. 2017. JCI Insight. 2:e93684. PubMed
  24. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  25. Weldon A, et al. 2015. J Rheumatol. 42:749. PubMed
  26. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  27. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  28. Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed
  29. Zabaleta N, et al. 2021. Cell Host Microbe. :. PubMed
  30. Port JR, et al. 2020. J Virol. 94:. PubMed
  31. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  32. Ponath V, et al. 2021. Int J Mol Sci. 22:. PubMed
  33. Levy Y, et al. 2021. iScience. 24:102711. PubMed
  34. Ireland RE, et al. 2022. Viruses. 14:. PubMed
  35. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  36. Dai HS et al. 2017. Immunity. 47(1):159-170 . PubMed
  37. Felices M, et al. 2018. JCI Insight. 3. PubMed
  38. Buters TP, et al. 2021. Br J Clin Pharmacol. Online ahead of print. PubMed
  39. Lu H, et al. 2020. Am J Reprod Immunol. 83:. PubMed
  40. Romani B, et al. 2016. J Biol Chem. 291: 2696 - 2711. PubMed
  41. Frasca D, et al. 2018. PLoS One. 13:e0197472. PubMed
  42. Wiedemann A, et al. 2020. Nat Commun. 3.048611111. PubMed
  43. Foote JR, et al. 2019. Front Immunol. 10:188. PubMed
  44. Ehlers L, et al. 2022. Int J Mol Sci. 23:. PubMed
  45. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  46. Flak MB, et al. 2020. J Clin Invest. 130:359. PubMed
  47. Vallera D, et al. 2016. Clin Cancer Res. 22: 3440 - 3450. PubMed
  48. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
  49. Friedensohn S, et al. 2018. Front Immunol. 9:1401. PubMed
RRID
AB_314217 (BioLegend Cat. No. 302017) AB_314218 (BioLegend Cat. No. 302018)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

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